만성 기도질환에서 흰쥐 뮤신에 대한 단 세포군 항체(RTO3)를 이용한 뮤신 측정에 관한 연구
- Alternative Title
- Measurement of Mucin Amounts Using RTO3 in Patients with Chronic Airway Disease
- Abstract
- Background: It has been anticipated that the amount and composition of mucin are changed in patients with chronic airway diseases. We evaluated whether RTO3(mAb against rat tracheal mucins) could quantify the amount of mucin from the airway in the patients with chronic airway diseases. Methods and results; 1) RTO3 was bound to high molecular weight of mucin based on Western blot in sputum and BALF from patients with chronic airway diseases. 2) The goblet cells and submucosal glands in main bronchus from human were observed by PAS stain. And immunohistochemical stain with RTO3 showed immunoreactivity on some goblet cells. 3) The amount of mucin was more increased in patients with chronic airway diseases compared to those in normal subjects. 4) In the exacerbation of asthmatics, mucin amounts were more increased than stable asthmatics. Conclusion: We suggested that secreted mucin in chronic airway diseases can be quantified by ELISA with RTO3.
- All Author(s)
- 김도진
; 김기업
; 남궁은경
; 어수택
; 김용훈
; 신찬영
; 고광호
; 박춘식
- Issued Date
- 1999
- Type
- Article
- Keyword
- Airway; MAbRTO3; Mucin
- Publisher
- 대한결핵및호흡기학회
- ISSN
- 0378-0066
; 1738-3536
; 2005-6184
- Citation Title
- 결핵 및 호흡기질환
Tuberculosis and Respiratory Disease
- Citation Volume
- 47
- Citation Number
- 6
- Citation Start Page
- 786
- Citation End Page
- 796
- Language(ISO)
- kor
- DOI
- 10.4046/trd.1999.47.6.786
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/677
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