Relationship between pulmonary surfactant protein and lipid peroxidation in lung injury due to paraquat intoxication in rats
- Abstract
- Background
Pulmonary damage resulting from lipid peroxidation is a principal effect of paraquat intoxication. The host-defense functions of surfactant are known to be mediated by the surfactant proteins A and D (SP-A and SP-D, respectively). The primary objective of this study was to evaluate the variations over time in levels of surfactant protein and lipid peroxidation (LPO) in lung tissue following free-radical-induced injury.
Methods
42 adult, male, Sprague-Dawley rats were administered intraperitoneal injections of paraquat (35 mg/kg body weight). SP-A and SP-D levels were determined via Western blot. LPO in the left lung homogenate was measured via analyses of the levels of thiobarbituric acid-reactive substances.
Results
LPO levels peaked at 6 hours, with no associated histological changes. SP-D levels increased until hour 12 and declined until hour 48; SP-D levels subsequently began to increase again, peaking at hour 72. SP-A levels peaked at hour 6, declining thereafter.
Conclusions
We suggest that in the early phase of paraquat injury, SP-D levels reflect alveolar damage and that de novo synthesis of SP-D takes 72 hours. Levels of SP-A, on the other hand, reflect abnormalities in the surfactant system in the late stage of paraquat intoxication. Surfactant proteins may play a role in protecting the lungs from reactive oxygen injury. A time-dependent variation has been observed in the levels of surfactant proteins A and D following paraquat injury, and it has been suggested that these proteins play a role in the protection of lung tissue against ROS-induced injuries.
- All Author(s)
- H. W. Gil
; M. H. Oh
; K. M. Woo
; E. Y. Lee
; S. Y. Hong
- Issued Date
- 2007
- Type
- Article
- Keyword
- Paraquat; Lipid peroxidation; Surfactant protein A; Surfactant protein D
- Publisher
- 대한내과학회
Korean Association of Internal Medicine
- ISSN
- 1226-3303
; 2005-6648
- Citation Title
- The Korean journal of internal medicine
- Citation Volume
- 22
- Citation Number
- 2
- Citation Start Page
- 67
- Citation End Page
- 72
- Language(ISO)
- eng
- DOI
- 10.3904/kjim.2007.22.2.67
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/3255
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