Characterization of aberrant FHIT transcripts in gastric adenocarcinomas
- Abstract
- Aberrant transcripts of FHIT (fragile histidine triad) have been reported in several types of primary tumors and cell lines, including gastric carcinoma. The role of these aberrant transcripts in tumorigenesis is not clear yet. Forty-eight aberrant-sized FHIT transcripts with various lengths and number in 35 cases of gastric adenocarcinomas were further characterized. Aberrant transcripts, with deletions and/or insertions, were frequently observed in 20 cases of tumors. Sequence analysis demonstrated that different types of aberrant transcripts used normal splice sites but skipped exons, contained the inserts with the part of intron 5 sequences, or used the FHIT cDNA sequence 179-180 as a cryptic splice acceptor site. Most of aberrant transcripts lacked exon 5 and were presumably non-functional as the translation initiation codon is located in exon 5. Additionally, other transcripts, indicative of additional splice processing, either deletions or insertions, were expressed in several tumors. Taken together, our data indicate that the FHIT gene expression is frequently altered in gastric adenocarcinomas by aberrant splicing, and suggest that different types of aberrant transcripts may result during the multi-step splice processing.
- All Author(s)
- S. H. Lee
; C. J. Kim
; H. K. Park
; J. W. Koh
; M. H. Cho
; M. J. Baek
; M. S. Lee
- Issued Date
- 2001
- Type
- Article
- Keyword
- Fhit; gastric adenocarcinoma; aberrant transcript; carcinogenesis; splicing fidelity
- Publisher
- 생화학분자생물학회
- ISSN
- 1226-3613
- Citation Title
- Experimental & Molecular Medicine
- Citation Volume
- 33
- Citation Number
- 3
- Citation Start Page
- 124
- Citation End Page
- 130
- Language(ISO)
- eng
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2658
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.