Oncogenic function of angiopoietin-2 in vitro and its modulation of tumor progression in colorectal carcinoma
- Abstract
- Angiopoietin-2 (Ang-2) has been investigated in cancer primarily in terms of its angiogenic function, and its role as an oncogene has yet to be elucidated. The current study hypothesized that Ang-2 may be an oncogene and have a function in tumor progression. An investigation of the function of Ang-2 in the LoVo colorectal cancer (CRC) cell line in vitro, which expresses a high level of Ang-2, was performed by knocking down endogenous expression with a targeted short hairpin RNA. The aggressive phenotypic effects of Ang-2 on experimental and control group cells were assessed using cell proliferation, migration and invasion assays. The association between Ang-2 expression levels and clinicopathological factors was evaluated in 415 CRC tissues using immunohistochemistry. Suppressing Ang-2 expression decreased cellular proliferation, invasion and migration in an in vitro study. Ang-2 overexpression was observed in 46% of patients with CRC and was significantly associated with pT (P=0.048), pN (P<0.001), venous invasion (P=0.023), lymphatic invasion (P<0.001) and tumor-node-metastasis stage (P=0.022). Furthermore, Ang-2 overexpression was an independent prognostic factor in pN stages 1 and 2. These results reveal that Ang-2 may be an oncogene in colorectal carcinogenesis and its expression may exert aggressive phenotypic effects during tumor progression. In addition, Ang-2 expression may serve as a prognostic marker and a potential drug target.
- All Author(s)
- H. Kim
; T. S. Ahn
; C. J. Kim
; S. B. Bae
; H. J. Kim
; C. S. Lee
; T. H. Kim
; J. Im
; S. H. Lee
; M. W. Son
; M. S. Lee
; M. J. Baek
; D. Jeong
- Issued Date
- 2017
- Type
- Article
- Keyword
- Angiopoietin-2; Colorectal carcinoma; Oncogenic function; shRNA
- Publisher
- Spandidos Publications
- ISSN
- 1792-1074
; 1792-1082
- Citation Title
- Oncology letters
- Citation Volume
- 14
- Citation Number
- 1
- Citation Start Page
- 553
- Citation End Page
- 560
- Language(ISO)
- eng
- DOI
- 10.3892/ol.2017.6203
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2550
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