The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells
- Abstract
- Objectives/hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation.
Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol.
Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC50) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation.
Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.
- All Author(s)
- J. D. Lee
; J. Y. Lee
; B. J. Baek
; B. D. Lee
; Y. W. Koh
; W. S. Lee
; Y. J. Lee
; B. M. Kwon
- Issued Date
- 2012
- Type
- Article
- Keyword
- Honokiol; vestibular schwannoma; apoptosis; ERK
- Publisher
- American Otological Society
American Laryngological Rhinological and Otological Society
American Laryngological Association
- ISSN
- 0023-852x
; 1531-4995
- Citation Title
- The Laryngoscope
- Citation Volume
- 122
- Citation Number
- 1
- Citation Start Page
- 162
- Citation End Page
- 166
- Language(ISO)
- eng
- DOI
- 10.1002/lary.21781
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/2379
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.