Comparison of PNA probe-based real-time PCR and Cobas TaqMan MTB for detection of MTBC
- Abstract
- We compared the sensitivities and specificities of peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR (TM) TB/NTM) and Cobas TaqMan MTB assays for detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. A total of 425 clinical specimens including 360 respiratory specimens and 65 non-respiratory specimens were evaluated for comparative analysis. In respiratory specimens, the sensitivity of TaqMan MTB and PNAqPCR assay for detection of MTBC was 82.9% and 91.5%, respectively. In non-respiratory specimens, the sensitivity of the TaqMan MTB and PNAqPCR assay was 23.1% and 76.9%, respectively. Overall, the sensitivity and specificity of the TaqMan MTB assay for detection of MTBC was 76.9% and 100%, respectively. The PNAqPCR assay had a sensitivity and specificity of 90% and 99.7%, respectively.
- All Author(s)
- J. W. Kim
; Y. J. Choi
; H. J. Kim
; J. S. Park
; H. S. Nam
; Y. Hwangbo
; D. U. Kim
; K. S. Park
- Issued Date
- 2013
- Type
- Article
- Keyword
- Peptide nucleic acids; PCR; Mycobacterium tuberculosis complex; Tuberculosis; Real-time PCR
- Publisher
- 한국바이오칩학회
- ISSN
- 1976-0280
- Citation Title
- Biochip Journal
- Citation Volume
- 7
- Citation Number
- 2
- Citation Start Page
- 85
- Citation End Page
- 88
- Language(ISO)
- eng
- DOI
- 10.1007/s13206-013-7201-0
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/1549
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