Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens
- Abstract
- Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII. 3 and 75.7% (28/37) for GII. 4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
- All Author(s)
- K. S. Park
; K. A. Baek
; D. U. Kim
; K. S. Kwon
; S. H. Bing
; J. S. Park
; H. S. Nam
; S. H. Lee
; Y. J. Choi
- Issued Date
- 2012
- Type
- Article
- Keyword
- Immunochromatographic assay; Norovirus; Sensitivity; Specificity
- Publisher
- 대한진단검사의학회
- ISSN
- 2234-3806
- Citation Title
- Annals of Laboratory Medicine
- Citation Volume
- 32
- Citation Number
- 1
- Citation Start Page
- 79
- Citation End Page
- 81
- Language(ISO)
- eng
- DOI
- 10.3343/alm.2012.32.1.79
- URI
- http://schca-ir.schmc.ac.kr/handle/2022.oak/1436
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